1967 Sep; 121 (3):596–608. Cazzulo JJ, Stoppani AO. 2012 Mar 15;519(2):118-30. doi: 10.1016/j.abb.2011.11.015. Since plant phosphopyruvate carboxylase (PEPC) was last reviewed in the over a decade ago (O'Leary 1982), significant advances have been made in our knowledge of this oligomeric, cytosolic enzyme. Biotin is initially carboxylated at the BC active site by ATP and bicarbonate. Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate … In 11 Ojibwa and 2 Cree patients with type A pyruvate carboxylase deficiency, Carbone et al. Regulation of Pyruvate Carboxylase Activity by Calcium in Intact Rat Liver Mitochondriae (Received for publication, July 10, 19GS) GEORGE A. I