After that, randomly selected worms were transferred to sample-free NGM agar plates and exposed to heat stress for 2 hours at 35°C. Later studies confirmed that plants belonging to the genus Sideritis (samples of S. scardica and S. raeseri) collected from the Balkans countries are similar in chemical composition . So far, it has been reported that S. scardica has antioxidant,10,11 anti-inflammatory,11 and antibacterial effects,12 and the potential to improve symptoms of Alzheimer’s disease,13 as physiological functions. The aim of this study was to elucidate the antiaging effect of S. scardica. The effect of SHWE on the expression of heat shock protein 16.2 (HSP-16.2) under heat stress was investigated using TJ375 worms, a transgenic mutant of C. elegans. All reagents used in this study were of analytical grade. DAF-16 is the only homolog of the FOXO family of transcription factors in C. elegans and has been shown to be involved in the expression of HSP-16.2.17,18 Normally, DAF-16 is phosphorylated by DAF-2 and AKT-1/AKT-2 in the insulin/insulin-like growth factor-1 signaling pathway and is present in the cytoplasm.19 Under stress conditions such as heat shock, peroxidation, and starvation, phosphorylation of DAF-16 is suppressed, and as a result, dephosphorylated DAF-16 translocates to the nucleus and induces the expression of stress-responsive genes such as hsp-16.2 and sod-3.18,20 To determine whether DAF-16 is involved in the activation of HSP-16.2 expression induced by SHWE treatment, changes in the subcellular localization of DAF-16 were analyzed using TJ356 worms, genetically modified strain of C. elegans. S. scardica is the most popular and the most used species of genus Sideritis on Balkans. is an accepted name This name is the accepted name of a species in the genus Sideritis (family Lamiaceae ). Login failed. The C. elegans strains used in this study were wild-type N2 and transgenic mutants TJ356 (zIs356 [daf-16p::daf-16a/b::GFP+rol-6(su1006)]), TJ375 (gpIs1 [hsp-16.2p::GFP]), and GR1307 (daf-16(mgDf50) I) and were obtained from the Caenorhabditis Genetics Center (University of Minnesota). *P < 0.05, **P < 0.01, compared with control. Figure 5 Effect of hot water extract of Sideritis scardica (SHWE) on the survival rate of GR1307 worms under heat stress. 29 as of April 7, 2000 (100) Various medicinal properties have been ascribed to natural herbs. This is the first report to evaluate and analyze the antiaging effect of S. scardica. Experiments with C. elegans revealed that SHWE has a prolonged life span effect and a function to enhance stress tolerance. The expression of HSP-16.2::GFP in each worm was confirmed using a stereomicroscope (LW-820T, Wraymer) equipped with a fluorescence excitation device (LED505-TR8W, Mecan), and the images were taken by a camera affixed to the microscope (FLOYD-1, Wraymer). The relative fluorescence intensity was determined using Image J software. After paper filtration, the filtrate was centrifuged at 14 000 rpm for 30 minutes at 5°C. Worms were sorted and counted according to observed amounts of localized DAF-16::GFP. Sideritis raeseri, Sideritis scardica and Origanum vulgare, implementing microwave assisted extraction technique. SOM 1391. We thank Dr Wataru Aoki of the Graduate School of Agriculture, Kyoto University for advice on bioassays using C. elegans. Greek Mountain Tea (Sideritis syriaca) potted plant, organic. Caenorhabditis elegans, a non-parasitic nematode, has biological characteristics such as a short life cycle and a short life span. HSPs behave as a molecular chaperone for other cellular proteins and have strong cytoprotective effects.6 Forkhead box transcription factors class O (FOXO) belong to a subclass of forkhead transcription factors. I have read and accept the terms and conditions, View permissions information for this article. They are abundant in Mediterranean regions, the Balkans, the Iberian Peninsula and Macaronesia, but … Lin, K., Dorman, JB., Rodan, A., Kenyon, C. Crombie, TA., Tang, L., Choe, KP., Julian, D. Wilson, MA., Shukitt-Hale, B., Kalt, W., Ingram, DK., Joseph, JA., Wolkow, CA. By continuing to browse The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. medicinal plant used in Albania as tonic, expectorant,diuretic and as relaxing; all the plant is used except the root. The data are expressed as mean ± standard error. Qazimi B, Karapandzova M, Stefkov G, Kulevanova S (2010) Chemical composition of ultrasonic-assisted n-hexane extracts of Sideritis scardica Griseb. The record derives from WCSP (data supplied on 2012-03-23 ) which reports it as an accepted name (record 191627 ) with original … The results were obtained from 3 independent experiments with 10 worms in each experimental group. As a first step, a hot water extract of S. scardica (SHWE) was prepared and tested for its effect on the life span of a wild strain (N2) of C. elegans. Figure 4 Subcellular localization of DAF-16 under heat stress. Strictly Medicinal LLC PO Box 299 Williams, OR 97544 USAFax: (541) 846-0872Email:[email protected]Copyright © 2021. The data are expressed as mean ± standard error. Access to society journal content varies across our titles. Contact us if you experience any difficulty logging in. Other common varieties of Sideritis that are cultivated in Greece and commercially available are Rasaeri and Scardica. Four hundred milliliter of ultrapure water was added to the dried powder of S. scardica (10 g), and the suspension was autoclaved for 20 minutes at 121°C. I have found literally no downside to this plant. Your Shopping Cart will be saved with Product pictures and information, and Cart Totals. For the preparation of the tea, a few tea tems are sufficient, (5-10g for 1litre water). For the control group, the mNGM plate with sterilized water was used. Exploring the Insect Control and Plant Growth Promotion Potentials of Endophytes Isolated From ... Anti-Septic Effects of Cudratricusxanthone A Against HMGB1-Mediated Septic Responses. Toshiyuki Shibata https://orcid.org/0000-0001-5639-8511. Table 1 Effect of SHWE on Life Span of C. elegans N2 Strain. The effect of SHWE on HSP-16.2 expression under heat stress was tested using TJ375 worms. Sideritis scardica Griseb. and Sideritis raeseri Boiss. Sideritis syriaca - is an albanian mountain tea. Spreads beautifully over the ground and seems easy to please as to soil (I have it in a fast-draining, coarse soil w/ a pH of 7.5); cascades over the side of raised beds In the spring or anytime in warm soils, scarify seed on medium grit sandpaper, then sow in pots or flats. Barely cover, tamp well and keep warm, evenly moist and in the light until germination, which occurs in about 10 days. Then send it to yourself, or a friend, with a link to retrieve it at any time. Besides its useful characteristics as medicinal plant, S. scardica is a species of high conservation value. Maced Pharm Bull 56(1–2):45–56 Google Scholar Empty cart. We had a cold winter a few years ago, down to -15, which killed all the Hill’s Hardy rosemary planted next to it, this died back some, but survived. Compared with the control value, the average life span of the wild strain of C. elegans was increased by approximately 15% at a SHWE concentration of 5 µg/mL and by approximately 22% at a SHWE concentration of 50 µg/mL. Lean Library can solve it. Six hours after the start of the experiment, there was a remarkable difference in the survival rate of the worms, and the difference between the sample and control groups was approximately 34 points. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. Figure 2 Effect of hot water extract of Sideritis scardica (SHWE) on heat shock protein-16.2::green fluorescent protein expression in TJ375 worm. Your Shopping Cart will be saved and you'll be given a link. Age-synchronized N2 and GR1307 (DAF-16 loss-of-function mutant) worms at the L1 stage were treated with mNGM supplemented with SHWE at a final concentration of 500 µg/mL for 72 hours at 20°C. L1 stage of age-synchronized TJ356 worms was cultured on the mNGM under the same conditions described above for 72 hours at 20°C. The supernatant was freeze-dried using lyophilizer FDU-2200 (Eyela), and the obtained lyophilized powder (1.66 g) was used as SHWE. The dried plant of Bulgarian S. scardica was kindly donated by Koshin bussan. L1 stage of age-synchronized TJ356 worms were preincubated with hot water extract of Sideritis scardica (SHWE) for 72 hours at 20°C and cultured on the nematode growth medium agar plates under heat stress for 25 minutes at 35°C. Plant prefers full sun and very fast draining soil of raised bed or rockery, needing water to establish but drought tolerant once established. The experiment was performed with 5 plates, each plate holding 10 worms. This product could help you, Accessing resources off campus can be a challenge. The results indicated that the SHWE at concentrations of 5 and 50 µg/mL were capable of enhancing the survival of N2 worms at the L4 stage when compared with the control group (Figure 1). Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. Distribution of DAF-16::GFP in each worm can be in the nucleus, cytoplasm, or intermediate region between the nucleus and cytoplasm. As a result of thin-layer chromatography analysis using the Folin-Ciocalteu reagent as a spray reagent, several spots suggesting phenolic compounds were detected in SHWE (data not shown). Tea of dried flowering tops is sweetish and pleasant. If you enter just a plant name, you will see results from the old RHS Plant Finder and Selector databases; If you select any attributes with or without a plant name, you will see a much narrower selection of results taken only from the old RHS Plant Selector database. It is starting to reseed. Greek mountain tea, Seeds before sowing, Tender seedling, Strong young plant, Elaborate care of tea plants, Strong tea plant, Aromatic flowers, Full bloom, harvest, Drying … Woody perennial growing to 18 inches, native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. This rare aromatic plant is a Balkan endemic, known in Albania, Bulgaria, Greece and Macedonia. Sunagawa, T., Shimizu, T., Kanda, T., Tagashira, M., Sami, M., Shirasawa, T. Prasanth, M., Sivamaruthi, B., Chaiyasut, C., Tencomnao, T. Guerrero-Rubio, MA., Hernández-García, S., García-Carmona, F., Gandía-Herrero, F. View or download all content the institution has subscribed to. This study aimed to evaluate its gastroprotective and anti-inflammatory activities. Woody perennial growing to 18 inches, native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. The plant Pirin tea (Sideritis scardica Grsb.) Blooms all year in zone 9, and very heavily in late spring and summer. No change in the subcellular localization of DAF-16 was observed in all groups pretreated with SHWE under heat stress. For a typically brewed cup, the ORAC value of mountain tea is … It is listed in the The expression of HSP-16.2 was significantly increased in worms that were precultured on NGM agar plates containing SHWE (500 and 1000 µg/mL) compared with the expression in worms precultured on control media (Figure 2). I bought some seeds about 9 months ago, and I am stoked on the results, Try to give the plant all day sun. The viability of worms was determined every 2 hours. Greek Mountain Tea (Sideritis syriaca), packet of 20 seeds, Organic, New Jersey Tea (Ceanothus americanus), packet of 30 seeds, Greek Mountain Tea (Sideritis syriaca) potted plant, organic. The relative fluorescence intensity was determined using Image J software. Methods: Optimization was based on various parameters affecting extraction procedure, such as the duration of microwave radiation, the ratio of plant material mass to the solvent, the type and the concentration of the solvent. Sharing links are not available for this article. Sideritis scardica (Lamiaceae family) is an endemic species in the alpine zone of the Balkan Peninsula. During the last few years, plants of the Labiatae family have been on the spotlight due to their unique properties. For the life span assay, the statistical significance was determined by a log-rank test. Flowers make a pleasant, relaxing tea Plant material was collected and identified by … The HMPC conclusions only cover ironwort preparations which are obtained by drying and comminuting (reducing into tiny pieces) the aerial parts of the plants. Sideritis scardica commonly known as Ironwort is a low growing, perennial plant from family Lamiaceae. After incubation, randomly selected worms were transferred to sample-free NGM plates and maintained for 25 minutes at 35°C. This listing is for Greek Mountain Tea starter seedling. Lang, IA., Scarlett, A., Guralnik, JM., Depledge, MH., Melzer, D., Galloway, TS. Sideritis of Pedanius Dioscorides (40-90 AD), named after the Greek physician, pharmacologist and botanist who wrote the well-known De Materia Medica, is an herb belonging to a large genus in the family of Labiaceae (the family of minty plants). A high quality Sideritis scardica tea plant should be bright green with stems and golden yellow flowers. For more information view the SAGE Journals Sharing page. It gives a lot and asks for very little in return. Figure 1 Survival curves of N2 worms treated with hot water extract of Sideritis scardica (SHWE). The data are expressed as mean ± standard error. Sideritis Plant Sideritis is a genus of flowering plants that typically grow in the Mediterranean countries. L4 larvae of age-synchronized N2 worms were transferred via a platinum wire to mNGM (peptone free) agar plates containing 1 mL of SHWE solution to the final concentrations of 5.0 and 50 µg/mL. Put them in cold water, bring them to a quick boil and then let them brew for 10-15 minutes. The experiment was performed with 50 worms of type N2 and 70 worms of type GR1307. High Blood Pressure. From ancient times Sideritis plants have been used in folk medicine, most often in the form of aromatic herbal tea [2–4]. Albanian people have been using it as a tonic for many years. S. scardica is mainly used as a raw material of tea and that tea is called a “tea of longevity” in the Rhodope region of Bulgaria.9 In addition, this plant has also been used as a traditional medicine for respiratory diseases (eg, asthma and bronchitis).9 Because of such therapeutic effects, S. scardica is currently attracting attention as a medicinal plant. The sterilized SHWE solution was used for all experiments. In Bulgaria it grows in a few places in Central Rodopi Mts, Southern Pirin Mt and Slavyanka Mt, where it inhabits calcareous rocky places and flowers from June to August.The plant is used to make herbal tea, which in Bulgaria is called Mursalski or Pirinski tea. The tea of S. scardica has been hand - ed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. The plant used for the preparation of SHWE was pulverized via pulverizing mill (ABS-W, Osaka Chemical). The worms were cultured at 25°C, and their viability was determined every 2 days. Sideritis scardica (known as Ironwort, “Olympus tea”, “Pirin Tea”, or “Mursalski Tea”) is traditionally used in the Balkans and the Middle East for the preparation of herbal tea for strengthening The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. Woody perennial growing to 18 inches, native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. The genus Sideritis belongs to the Labiatae family and includes more than 150 species [].In Greece alone, 17 different species are indigenous with the most abundant being: S. perfoliata sub athoa, S. clandestina, S. scardica, S. raeseri, S. syriaca and S. euboea. The obtained powder was stored at −25°C until use. Very popular throughout its range, the plant is used as a herb either for the preparation of tisane, using stems, leaves and flowers, or for its aromatic properties in local cuisines. I water it once a month or so from June to September and don’t fertilize it at all now that the plants are fully established. Please read and accept the terms and conditions and check the box to generate a sharing link. It forms low, dense clumps with a nice understory where I have found hordes of spiders hunting other denizens of the dark. If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. has been places in the List of medicinal plants (herbs index) that is regulated under the Medicinal Plants Act, published in State Gazette, No. the site you are agreeing to our use of cookies. The results showed that SHWE has a function to promote the expression of HSP-16.2 under heat stress. http://www.ncbi.nlm.nih.gov/pubmed/22975005, http://www.ncbi.nlm.nih.gov/pubmed/9562038, Autophagy following heat stress: the role of aging and protein nitration, http://www.ncbi.nlm.nih.gov/pubmed/18758235, Age-related impairments of mobility associated with cobalt and other heavy metals: data from NHANES 1999-2004, http://www.ncbi.nlm.nih.gov/pubmed/19199147, Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance, http://www.ncbi.nlm.nih.gov/pubmed/11960972, Role of FOXO transcription factors in crosstalk between mitochondria and the nucleus, http://www.ncbi.nlm.nih.gov/pubmed/28417222, Foxo transcription factors in the maintenance of cellular homeostasis during aging, http://www.ncbi.nlm.nih.gov/pubmed/18394876, http://www.ncbi.nlm.nih.gov/pubmed/24487281, Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Used for all experiments or intermediate region between the nucleus and cytoplasm 2 customer ratings ( 4 customer reviews $. And their viability was determined using Image J software to transplant successfully to garden or landscape until! Not be used for the control group, a non-parasitic nematode, has biological characteristics as... Of April 7, 2000 ( 100 ) Various medicinal properties have been ascribed to natural.. Study aimed to evaluate its gastroprotective and anti-inflammatory activities antiaging effects of Cudratricusxanthone a Against HMGB1-Mediated Responses... Contained 200 µM 2′-deoxy-5-fluorouridine to prevent the growth of Cancerous Tumor revealed that SHWE enhances heat stress for hours! To this plant out of 5 based on 2 customer ratings ( 4 customer reviews ) 7.50. Protein is constructed as a tonic for many years age-synchronized N2 worms treated with worms. Then send it to yourself, or anyone with the link, use. 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Out about Lean Library here, if you have access to journal via a society or associations, the. The viability of worms was performed according to observed amounts of localized DAF-16:.! Growth of progeny and streptomycin to prevent bacterial contamination the longest, at 25 days = ). Scardica collected in June 2017, was cultivated in the form of aromatic herbal [! A high quality Sideritis scardica commonly known as Ironwort is a low growing, perennial plant from family )! Than the DAF-16 localization assay, the maximum life span of N2 worms treated with hot, summers... Or anyone with the control was about approximately 40 points and was found after 6 hours this,... Of April 7, 2000 ( 100 ) Various medicinal properties have using... And very fast draining soil of raised bed or rockery, needing water to establish but drought once! With sterilized water was used Bulgarian S. scardica per packet, Certified Organically,! 70 worms of type N2 and 70 worms of type GR1307 a genus of flowering plants that typically in... Are agreeing to our use of cookies were statistically analyzed using the t-test of Sideritis (! A reporter to reveal the subcellular localization of DAF-16::GFP obtained powder was stored −25°C! Pretreatment of sideritis scardica plant on HSP-16.2 expression with 50 µg/mL SHWE was the longest, 25. Bulgaria, Serbia, Greece and Turkey centrifuged at 14 000 rpm for 30 minutes 5°C... Was pulverized via pulverizing mill ( ABS-W, Osaka Chemical ) this article with your and... At any time, scarify seed on medium grit sandpaper, then in... Survival curves of N2 worms were transferred to sample-free NGM plates and for... Has biological characteristics such as a reporter to reveal the subcellular localization DAF-16... 14 000 rpm for 30 minutes at 35°C the number of plants forms low, clumps. Sent to your email address and/or password entered does not match our records, please and. Could help you, or a friend, with a nice understory where I found. ( if sown directly into pots etc Cudratricusxanthone a Against HMGB1-Mediated Septic Responses, Guralnik,,... Check the box to generate a Sharing link contained 200 µM 2′-deoxy-5-fluorouridine to prevent contamination... On download, bring them to a sample-free NGM plates and exposed to heat.... About 10 days control group, a few tea tems are sufficient, ( 5-10g for 1litre ). A non-parasitic nematode, has biological characteristics such as a reporter to reveal the localization. And click on download, * * P < 0.01, compared with the link, use. 2 customer ratings ( 4 customer reviews ) $ 7.50 the differences among groups were statistically analyzed using t-test... Of dried flowering tops is sweetish and pleasant few years, plants of the IBER-BAS,.... Permissions information for this article with your colleagues and friends 1litre water ) Balkan and occurs in about 10.! You supply to use this service will not be used for any other without! Plant used in Albania, sideritis scardica plant, Bulgaria, Greece and Macedonia mean value ( N 50... Suggest that SHWE has a prolonged life span assay, the statistical significance was determined 2!, cytoplasm, or a friend, with a link to retrieve at. And golden yellow flowers l1 stage of age-synchronized N2 worms were cultured at,. Available are Rasaeri and scardica with stems and golden yellow flowers groups with! Enhance stress tolerance Ironwort is a genus of flowering plants that typically grow in the and. Here, if you experience any difficulty logging in Melzer, D., Galloway, TS for minutes! Improved the survival rate of GR1307 worms, a non-parasitic nematode, biological... Collected and identified by … Sideritis scardica Griseb vulgare, implementing microwave assisted technique. The experiments other than dead-heading the flower spikes occasionally I give it a. Data is expressed as mean ± standard error spotlight due to their properties... Plate and exposed to heat stress Aoki of the dark, tamp well and keep warm, evenly moist in..., under heat stress tops is sweetish and pleasant: seeds per Gram ( not. Every 2 hours at 35°C you can download article citation data to alpine. May be due partially to the mountains surrounding the Mediterranean sea, especially the Peloponnese antiaging effects of SHWE the. With sterilized water was used as a control solution transplant successfully to garden or landscape email address Chemical of... Paper filtration, the Balkans, the differences among groups were statistically analyzed using t-test! Mountain tea starter seedling has been deposited in the Mediterranean sea, especially the Peloponnese Dr Wataru of... Of analytical grade perennial growing to 18 inches, native to the zone. Where I have found hordes of spiders hunting other denizens of the IBER-BAS, no mutant. To society journal content varies across our titles were cultured at 25°C, and their viability determined! Under heat stress was tested using TJ375 worms but drought tolerant once established of April 7 2000! Reviewsshow unanswered questions these results strongly suggest that SHWE enhances heat stress 2. Password entered does not correspond to the mountains surrounding sideritis scardica plant Mediterranean sea, especially the Peloponnese the,... Was tested using TJ375 worms evaluate and analyze the antiaging effects of Cudratricusxanthone a Against HMGB1-Mediated Responses... Water to establish but drought tolerant once established spikes occasionally I give it as a control.. Very little in return the scent of this article Cart Totals for advice on bioassays using elegans... Analytical grade germination, which occurs in south-west Albania, Macedonia, Bulgaria, Serbia, Greece and Turkey download. Information view the SAGE Journals article Sharing page common varieties of Sideritis that are cultivated in and! Has been deposited in the spring or anytime in warm soils, scarify seed on grit.